Using 16S rDNA Sequencing Technology to Preliminarily Analyze Intestinal Flora in Children with Mycoplasma pneumoniae Pneumonia / 生物医学与环境科学（英文）

Objective@#We investigated changes in the intestinal flora of children with Mycoplasma pneumoniae pneumonia (MPP).@*Methods@#Between September 2019 and November 2019, stool samples from 14 children with MPP from The Fourth Hospital of Baotou city, Inner Mongolia Autonomous Region, were collected and divided into general treatment (AF) and probiotic (AFY) groups, according to the treatment of "combined Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus cereus tablets live". High-throughput 16S rDNA sequencing was used to identify intestinal flora.@*Results@#Intestinal flora abundance and diversity in children with MPP were decreased. Both Shannon and Simpson indices were lower in the AF group when compared with healthy controls ( P < 0.05). When compared with healthy controls, the proportion of Enterorhabdus was lower in the AF group, while the proportion of Lachnoclostridium was higher ( P < 0.05). The proportion of Bifidobacteria and Akkermansia was lower in the AFY group but Enterococcus, Lachnoclostridium, Roseburia, and Erysipelatoclostridium proportions were higher. The proportion of Escherichia coli- Shigella in the AFY group after treatment was decreased ( P < 0.05).@*Conclusions@#The intestinal flora of children with MPP is disturbed, manifested as decreased abundance and diversity, and decreased Bifidobacteria. Our probiotic mixture partly improved intestinal flora disorders.

Clinical study of dynamic changes of T lymphocyte level in children with protracted bacterial bronchitis / 中国实用儿科杂志

OBJECTIVE: To investigate the relationship between pathogenesis of PBB and the T cell subsets disorders and to evaluate the clinical significance of immunomodulatory therapy to the prognosis of PBB and the prevention of recurrent PBB. METHODS: A total of 127 cases of PBB children treated in the Fourth Hospital of Baotou City from May2015 to May 2018 were selected. Blood samples were collected before treatment,and the levels of CD4,CD8 cells and CD4/CD8 in peripheral blood T cell subsets were detected by flow cytometry. All children with PBB(PBB group)were given oral amoxicillin and clavulanate potassium for 2 to 4 weeks. The subjects were divided into Huaiqihuang group(n=66)and non-Huaiqihuang group(n=66). The Huaiqihuang group was given Huaiqihuang granules based on the anti-infective treatment. In addition,healthy children were enrolled as control group(n=39). T cell subsets of the two groups were reexamined 3 months after treatment. By analyzing the cough symptom scores of all children after 2 weeks,4 weeks and 12 weeks of treatment,the rate of cure and improvement and the recurrence rate after 1 year were evaluated.SPSS16.0 software was used for data processing,and the difference was significant if P0.05). In 2 weeks after treatment,the cough symptom scores of the PBB group began to decrease,and the non-Huaiqihuang group decreased more significantly,with statistically significant difference(P0.05). Follow-up showed that the recurrence rate of Huaiqihuang group was lower than that of non-Huaiqihuang group in 1 year after the end of treatment,and the difference between the two groups was statistically significant(P<0.01). CONCLUSION: Children with PBB have T cell subsets disorder,and the treatment of anti-infection combined with Huaiqihuang granules for at least 4 weeks has a positive effect on PBB prognosis and recurrence prevention.

Epidemiological survey of childhood asthma in 2010 in urban Baotou, China / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the prevalence rate of childhood asthma in 2010 in urban Baotou, China, as well as the characteristics of attacks and the status of diagnosis and treatment of childhood asthma.</p><p><b>METHODS</b>More than 10 000 children (0-14 years) were selected from 3 secondary schools, 3 primary schools, 6 kindergartens, and 4 community vaccination sites in urban Baotou by cluster random sampling between September 2009 and August 2010. A standardized preliminary questionnaire was used for screening out suspected cases, which were then confirmed or excluded by a clinician; the confirmed cases underwent further questionnaire survey. Double entry and validation was adopted for all data using Epi-Info software, and analysis was performed using SPSS 13.0.</p><p><b>RESULTS</b>A total of 11 323 children were surveyed. Asthma was diagnosed in 127 cases (including 121 children with typical asthma and 6 children with cough variant asthma), with a prevalence rate of 1.12%. The prevalence rate of asthma in male children was significantly higher than that in female children (1.51% vs 0.72%; P<0.01). The prevalence rate of asthma in 2010 was significantly increased compared with that in 1990 (0.55%) and 2000 (0.88%) (P<0.05). Systemic glucocorticoid use decreased significantly from 60.2% in 2000 to 25.9% in 2010 (P<0.01); inhaled corticosteroid use increased significantly from 13.6% in 2000 to 85.8% in 2010 (P<0.01); antibiotic use decreased from 98.1% in 2000 to 66.9% in 2010 (P<0.01). The multivariate logistic regression analysis showed that family history of allergy, allergic rhinitis, chronic cough, and recurrent respiratory tract infection were independent risk factors for childhood asthma.</p><p><b>CONCLUSIONS</b>The prevalence rate of childhood asthma in urban Baotou shows an increasing trend. Inhaled corticosteroids have been widely used.</p>

Relationship between polymorphisms of IL-13 gene Intron 3 + 1923 and bronchial asthma in children / 中华实用儿科临床杂志

Objective To explore the correlation of the polymorphism of gene IL-13 Intron 3 + 1923and IL-13 and total IgE with pediatric asthma.Methods Polymerase chain reaction-restriction fragment length polymorphisms was used to analyze the polymorphism of gene IL-13 lntron 3 + 1923 in 50 normal children (healthy control group) and 77 asthma children (asthma group); enzyme linked immunosorbent assay was used to measure serum IL-13 and total IgE,and used the SPSS 18.0 software to analyze the serum IL-13 and total IgE in 2 groups.Results 1.The gene type and allele gene frequency distribution of gene IL-13 site Intron 3 + 1923 in asthma group and healthy control group had statistical significance(genotype:x2 =7.239,P ＜ 0.05 ; allele frequency:x2 =6.731,P ＜ 0.01),Allele T had a relationship with asthma.(2) The serum IgE concentration in asthma group was (50.98-± 27.97) mg/L,and in normal control group was (42.85 ± 27.19) mg/L,contrast difference had statistical significant (t =3.067,P ＜ 0.01).(3) The serum IgE in asthma group was (197.13 ±94.7) ng/L and in healthy control group was (159.06 ± 107.65) ng/L,contrast difference had statistical significant (t =2.079,P ＜ 0.05).(4) Different genotype of gene IL-13 Intron 3 + 1923 in asthma group had distinguishing level of IgE:CC was (30.25 ± 19.75) mg/L,CT was (57.50 ± 27.64) mg/L,TT was (65.09 ± 21.14) mg/L,group contrast had significant difference (P ＜ 0.05) ; Different genotype of gene IL-13 Intron 3 + 1923 in asthma group had distinguishing level of IL-13,CC:(105.59 ± 57.32) ng/L,CT:(222.45 ± 74.00) ng/L,TT:(274.38 ±86.12) ng/L,group contrast had significant difference (P＜0.05).Conclusions Polymorphism of gene IL-13 Tntron 3 + 1923 is relevant with children asthma,allele T gene is the correlative gene of children asthma.Allele IL-13 Intron 3 + 1923T has an influence on the level of IL-13,IgE,which makes them higher.Increasing total IL-13 and IgE in serum has a relationship with children asthma.

Influence of Naloxone on ?-Exdorphin and Calcitonin Gene-Related Peptide in Convulsive Children with Brain Damage / 实用儿科临床杂志

0.01),which were significantly different from group Ⅲ(P